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ALDH1L1 Antibody [ALDH1L1/7701]

In Stock
Catalog Number Formulation Size Price
10840-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10840-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10840-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified ADH1L1 Mouse Monoclonal Antibody (ALDH1L1/7701). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified ADH1L1 Mouse Monoclonal Antibody (ALDH1L1/7701). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of SK-BR3 cell lysate using ADH1L1 Mouse Monoclonal Antibody (ALDH1L1/7701).

Western blot analysis of SK-BR3 cell lysate using ADH1L1 Mouse Monoclonal Antibody (ALDH1L1/7701).

Formalin-fixed, paraffin-embedded human liver stained with ADH1L1 Mouse Monoclonal Antibody (ALDH1L1/7701). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human liver stained with ADH1L1 Mouse Monoclonal Antibody (ALDH1L1/7701). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde, lipid peroxidation and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH1L1 (aldehyde dehydrogenase 1 family member L1), also known as FTHFD or 10-FTHFD (10-formyltetrahydrofolate dehydrogenase), is a cytosolic protein that is developmentally regulated in the cerebellum. ALDH1L1 binds to folate and catalyzes the conversion of 10-formyltetrahydrofolate (10-FTHF) to tetrahydrofolate (THF). This suggests a possible role for ALDH1L1 in the regulation of cellular THF levels as well as in the inhibition of cell proliferation (as 10-FTHF is essential for the synthesis of purine). In addition, the overexpression of ALDH1L1 can restrict cell proliferation in vitro.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG /
Cellular Localization
Cytoplasm.
Gene Name
ALDH1L1
Positive Control
Human kidney or liver. A549 or SK-BR3 cells.
Immunogen
Recombinant fragment of human ALDH1L1 protein (exact sequence is proprietary)
Alternate Names
Cytosolic 10-formyltetrahydrofolate dehydrogenase, Aldehyde dehydrogenase family 1 member L1, 10-FTHFDH; Aldehyde dehydrogenase family 1 member L1; aldh1l1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; EC 1.5.1.6; FDH; formyltetrahydrofolate dehydrogenase; FTHFD

Database Links

Entrez Gene ID
10840
SwissProt
O75891

Additional Information

Clone
ALDH1L1/7701
Chromosome Location
3q21.3
Mol. Weight of Antigen
99kDa

Related Antibodies

View All ALDH1L1 Antibodies

Functions

  • Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:19933275, PubMed:21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity).

Key References

  • Champion, K.M., et al. 1994. Identification of a heritable deficiency of the folate-dependent enzyme 10-formyltetrahydrofolate dehydrogenase in mice. Proc. Natl. Acad. Sci. USA 91: 11338-11342.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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