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Recombinant PTEN (Tumor Suppressor Oncoprotein) Antibody [PTEN/12943R]

In Stock
Catalog Number Formulation Size Price
5728-RBM18-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5728-RBM18-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5728-RBM18-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
PTEN Antibody in Western Blot (WB)

PTEN Antibody in Western Blot (WB)

Western Blot Analysis of HeLa, DU 145 and A-431 cell lysates using PTEN Rabbit Recombinant Monoclonal Antibody (PTEN/12943R).

PTEN Antibody in SDS-PAGE

PTEN Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified PTEN Recombinant Rabbit Monoclonal Antibody (PTEN/12943R). Confirmation of Purity and Integrity of Antibody.

PTEN Antibody in Western Blot (WB)

PTEN Antibody in Western Blot (WB)

Western Blot Analysis of HeLa and Jurkat cell lysates using PTEN Recombinant Rabbit Monoclonal Antibody (PTEN/12943R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

PTEN (phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase) contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. It was identified as a tumor suppressor that is mutated in a large number of cancers, including sporadic brain, breast, kidney, and prostate cancers.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell projection, Cytoplasm, Dendritic spine, Nucleus, PML body, Postsynaptic density, Secreted
Gene Name
PTEN
Positive Control
Human prostate, Ovary, Heart, breast, liver or renal carcinoma. DU 145, A-431, MCF7 or HeLa, Jurkat.
Immunogen
Recombinant fragment (around aa392-403) of the human PTEN protein (exact sequence is proprietary)
Alternate Names
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, 10q23del|BZS|CWS1|DEC|GLM2|MHAM|MMAC1|PTEN1|PTENbeta|PTENgama|TEP1

Database Links

Entrez Gene ID
5728
SwissProt
P60484

Additional Information

Clone
PTEN/12943R
Chromosome Location
10q23.31
Mol. Weight of Antigen
47.17kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All PTEN Antibodies

Functions

  • Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins (PubMed:9187108, PubMed:9256433, PubMed:9616126). Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3 (PubMed:16824732, PubMed:26504226, PubMed:9593664, PubMed:9811831). Furthermore, this enzyme can also act as a cytosolic inositol 3-phosphatase acting on Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate (PubMed:11418101, PubMed:15979280). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (PubMed:31492966, PubMed:37279284). The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation (PubMed:11707428). In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement (PubMed:22279049). Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation (PubMed:22279049). Required for growth factor-induced epithelial cell migration; growth factor stimulation induces PTEN phosphorylation which changes its binding preference from the p85 regulatory subunit of the PI3K kinase complex to DLC1 and results in translocation of the PTEN-DLC1 complex to the posterior of migrating cells to promote RHOA activation (PubMed:26166433). Meanwhile, TNS3 switches binding preference from DLC1 to p85 and the TNS3-p85 complex translocates to the leading edge of migrating cells to activate RAC1 activation (PubMed:26166433). Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Involved in the regulation of synaptic function in excitatory hippocampal synapses. Recruited to the postsynaptic membrane upon NMDA receptor activation, is required for the modulation of synaptic activity during plasticity. Enhancement of lipid phosphatase activity is able to drive depression of AMPA receptor-mediated synaptic responses, activity required for NMDA receptor-dependent long-term depression (LTD) (By similarity). May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability (PubMed:10468583, PubMed:18716620)., Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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