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EGFL7 (Tumor Angiogenesis Marker) Antibody [EGFL7/9497]

Additional information

Catalog Number: 51162-MSM1
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
51162-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
51162-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
51162-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
EGFL7 Antibody in SDS-PAGE

EGFL7 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified EGFL7 Mouse Monoclonal Antibody (EGFL7/9497). Confirmation of Purity and Integrity of Antibody.

EGFL7 Antibody in Western Blot (WB)

EGFL7 Antibody in Western Blot (WB)

Western blot analysis of 293T cell lysate using EGFL7 Mouse Monoclonal Antibody (EGFL7/9497). Predicted band size: 30kDa / Observed band size: 20kDa

EGFL7 Antibody in Immunohistochemistry (IHC (P))

EGFL7 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with EGFL7 Mouse Monoclonal Antibody (EGFL7/9497). Inset: PBS instead of primary antibody; secondary only negative control.

EGFL7 Antibody in Western Blot (WB)

EGFL7 Antibody in Western Blot (WB)

Western blot analysis of human kidney, human lung, human spleen, HL60, K-562 and MCF7 lysates using EGFL7 Mouse Monoclonal Antibody (EGFL7/9497).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
EGFL7/9497
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Extracellular space, Secreted
Gene Name
EGFL7
Positive Control
293T cells. Human endometrium, tonsil, kidney or placenta. human kidney, human lung, human spleen, HL60, K-562 or MCF7.
Immunogen
Recombinant fragment (around aa1-200) of human EGFL7 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.
Key References
  • Larochelle C, Uphaus T, Broux B, et al.
  • de Oliveira C, Martins SFF, Gonçalves PG, et al.
  • Liu W, Zhang Y, Huang F, et al.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
51162
SwissProt
Q9UHF1
Chromosome Location
9q34.3
Mol. Weight of Antigen
30kDa
Alternate Names
epidermal growth factor-like protein 7, Epidermal growth factor-like protein 7, EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, NOTCH4-like protein, Vascular endothelial statin, Zneu1, Multiple EGF-like domains protein 7, VE-statin, EGFL7, UNQ187/PRO1449, MEGF7, MEGF7

Summary

Epidermal growth factor (EGF) repeat-containing proteins constitute an expanding family of proteins that are involved in several cellular activities, such as blood coagulation, fibrinolysis, cell adhesion and neural and vertebrate development. A human EGF repeat superfamily member that maps to human chromosome X, EGFL6, encodes a predicted signal peptide suggesting that it is secreted. EGFL6 is expressed in brain and lung tumors and fetal tissues but is generally absent from normal adult tissues. EGFL7 is a secreted protein that regulates vascular tubulogenesis in vivo. In vitro, EGFL7 inhibits platelet-derived growth factor induced smooth muscle cell migration and promotes adhesion of endothelial cells to the substrate. EGFL7 is expressed specifically by endothelial cells of the heart, lung and kidney.

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