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Cytokeratin (CAM5.2) (Epithelial Marker) Antibody [CAM5.2]

Additional information

Catalog Number: MSM62-13139
Reactivity:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
MSM62-13139-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
MSM62-13139-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
MSM62-13139-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin Antibody in Immunohistochemistry (IHC (P))

Cytokeratin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Cytokeratin Mouse Monoclonal Antibody (CAM5.2). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

[CAM5.2] Antibody in SDS-PAGE

[CAM5.2] Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Keratin, type II cytoskeletal 1b [CAM5.2] Monoclonal Antibody (CAM5.2). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
CAM5.2
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cytoplasm
Positive Control
Human colon, lung, prostate or breast.
Immunogen
Hybridization of mouse P3/NS-1/1-Ag4-1myeloma cells with spleen cells from BALB/c mice immunized with the human colorectal carcinoma cell line HT29.
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Key References
  • Albers et al. Am J Pathol 90: 17, 1988.
  • Ellis et al. J Clin Pathol 41: 1064, 1988. Oikawa et al. Biochem
  • Biophys Res Commun 144: 634, 1987.
  • Pfaltz et al. Virchows Arch A 411: 387, 1987.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
Not Known
SwissProt
N/A
Alternate Names
Keratin, type II cytoskeletal 1b, Cytokeratin-1B, Keratin-77, Type-II keratin Kb39, K1B; KRT1B; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B

Summary

Cytokeratin CAM5.2 antibody has a primary reactivity with human keratin proteins that correspond to Moll’s peptides #7 and #8, MW 48 and 52 kDa, respectively. Cytokeratin 7 and 8 are present in secretory epithelia of normal human tissue but not on stratified squamous epithelium.

Cytokeratin LMW CAM5.2 antibody stains most epithelial-derived tissue, including liver, renal tubular epithelium, and hepatocellular and renal cell carcinomas. Anti-Cytokeratin (CAM 5.2) may not react with some squamous cell carcinomas.

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