| Catalog Number: | 768-RBM17 |
|---|---|
| Gene: | |
| Host: | |
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Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with CA9 Recombinant Rabbit Monoclonal Antibody (CA9/9621R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis PurifiedCA9 Rabbit Recombinant Monoclonal Antibody (CA9/9621R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Stomach, Mouse Stomach and Rat Stomach tissue lysates using Carbonic Anhydrase IX Rabbit Monoclonal Antibody (CA9/9621R).
Western Blot Analysis of Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach and Guinea pig Stomach tissue lysates using Carbonic Anhydrase IX Rabbit Monoclonal Antibody (CA9/9621R).
Western Blot Analysis of Human Brain, Human Heart, Human Intestine, Human Kidney, Human Liver, Human Lung, Human Ovary, Human Pancreas, Human Prostrate, Human Skeletal Muscle, Human Skin, Human Spleen, Human Stomach and Human Testis tissue lysates using Carbonic Anhydrase IX Recombinant Rabbit Monoclonal Antibody (CA9/9621R).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman’s capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
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