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Recombinant CD21 (Mature B-Cell & Follicular Dendritic Cell Marker) Antibody [rCR2/9537]

In Stock
Catalog Number Formulation Size Price
1380-MSM22-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1380-MSM22-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1380-MSM22-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD21 Antibody in Immunohistochemistry (IHC (P)).

CD21 Antibody in Immunohistochemistry (IHC (P)).

Formalin-fixed, paraffin-embedded human tonsil stained with CD21 Recombinant Mouse Monoclonal Antibody (rCR2/9537). Inset: PBS instead of primary antibody; secondary only negative control.

CD21 Antibody in SDS-PAGE Analysis.

CD21 Antibody in SDS-PAGE Analysis.

SDS-PAGE Analysis Purified CD21 Recombinant Mouse Monoclonal Antibody (rCR2/9537). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 140kDa, which is identified as the complement receptor 2(CR2)/CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro.CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha.CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane
Gene Name
CR2
Positive Control
WEHI-231 cells. Human tonsil (IHC).
Immunogen
Recombinant fragment (around aa142-240) of the human CD21 protein (exact sequence is proprietary)
Alternate Names
Complement receptor type 2, C3DR|CD21|CR|CVID7|SLEB9

Database Links

Entrez Gene ID
1380
SwissProt
P20023

Additional Information

Clone
rCR2/9537
Chromosome Location
1q32.2
Mol. Weight of Antigen
112.91kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All CR2 Antibodies

Functions

  • Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1 (PubMed:1849076, PubMed:21527715, PubMed:7753047). When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells (PubMed:21527715). This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling (PubMed:1383329, PubMed:30107486). Also induces specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner (PubMed:12938232). Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production (PubMed:1386409)., (Microbial infection) Acts as a receptor for Epstein-Barr virus.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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