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GPRC5A / PEIG-1 / RAI3 / RAIG1 / TIG1 Antibody [GPRC5A/7893]

Additional information

Catalog Number: 9052-MSM1
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
9052-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
9052-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
9052-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
GPRC5A / PEIG-1 / RAI3 / RAIG1 / TIG1 Antibody

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GPRC5A Mouse Monoclonal Antibody (GPRC5A/7893). Inset: PBS instead of primary antibody; secondary only negative control.

GPRC5A / PEIG-1 / RAI3 / RAIG1 / TIG1 Antibody - Image 2

Formalin-fixed, paraffin-embedded human bladder stained with GPRC5A Mouse Monoclonal Antibody (GPRC5A/7893). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

GPRC5A Antibody in SDS-PAGE

GPRC5A Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Retinoic acid-induced protein 3 Mouse Monoclonal Antibody (GPRC5A/7893). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
GPRC5A/7893
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane, Cytoplasmic vesicle membrane
Gene Name
GPRC5A
Positive Control
Human lung or bladder.
Immunogen
Recombinant fragment (around aa200-357) of human GPRC5A protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity). May act as a lung tumor suppressor (PubMed:18000218).
Key References
  • Brauner-Osborne, H., et al. 2000. Sequence and expression pattern of a novel human orphan G protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain. Genomics 65: 121-128.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
9052
SwissProt
Q8NFJ5
Chromosome Location
12p13.1
Mol. Weight of Antigen
32kDa
Alternate Names
Retinoic acid-induced protein 3, G-protein coupled receptor family C group 5 member A, Phorbol ester induced gene 1, Retinoic acid-induced gene 1 protein, G-protein coupled receptor family C group 5 member A; Orphan G protein coupling receptor PEIG1; RAI 3; Raig1; Retinoic acid induced gene 1 protein; Retinoic acid induced protein 3; Retinoic acid responsive gene; Retinoic acid-induced protein 3

Summary

Retinoic acid-induced protein 3 (RAI3) is an transmembrane G protein-coupled receptor that affects many essential biological processes including embryogenesis, cell growth, differentiation, and apoptosis. RAI3 may also be involved in maintaining homeostasis of epithelial cells. Retinoic acid receptors directly regulate RAI3 during its transcription in embryonal carcinoma differentiation. RAI3 expression is upregulated in most tumor cell lines that express mutant p53, suggesting that p53 interacts with the promoter of RAI3 and represses its expression at the beginning of apoptosis. RAI3 is a potential molecular target for diagnosing breast cancer, and selective suppression of signals from RAI3 may have a place in breast cancer treatments.

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