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Formalin-fixed, paraffin-embedded human thyroid stained with S100A13 Mouse Monoclonal Antibody (S100A13/7484). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified S100A13 Mouse Monoclonal Antibody (S100A13/7484). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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The S-100 protein family consists of a group of calcium-binding proteins, that exhibit cell and tissue-specific expression. The expression levels of its members differ in various pathological conditions. The extracellular functions of the S-100 family may include the ability to enhance neurite outgrowth, involvement in inflammation and motility of tumor cells. S-100A13 is a 98 amino acid protein with 2 EF-hand calcium-binding domains. High levels of S-100A13 mRNA are present in skeletal muscle, heart, kidney, ovary, small intestine and pancreas. S-100A13 translocates in response to elevated intracellular calcium levels induced by Angiotensin II. S-100A13 co-localizes with S-100A1 on human chromosome 1q21.3, the site where the majority of S-100 proteins cluster.
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