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CD48 (Pan Leukocyte Marker) Antibody [5-4.8]

Additional information

Catalog Number: 962-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
962-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
962-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
962-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD48 (Pan Leukocyte Marker) Antibody

SDS-PAGE Analysis of Purified CD48 Mouse Monoclonal Antibody (5-4.8). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Clone
5-4.8
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
CD48
Positive Control
Daudi, Jurkat and human lymphocytes. Human lymph node and tonsil., JY, Raji
Immunogen
Human peripheral blood lymphocytes
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including 2B4/CD244 or CD2 to regulate immune cell function and activation (PubMed:27249817, PubMed:12007789). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:9841922, PubMed:27249817).
Key References
  • Vaughan HA et al. The isolation of cDNA clones for CD48. Immunogenetics 1991, 33(2):113-117.
  • Vaughan HA et al. Hu Ly-M3--a human leukocyte antigen. Transplantation 1983, 36(4):446-450.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
962
SwissProt
P09326
Chromosome Location
1q21.3-q22
Mol. Weight of Antigen
45kDa
Alternate Names
CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1, B cell membrane protein; B-cell activation marker; BCM1 surface antigen; BLAST1; Signaling lymphocytic activation molecule 2; SLAM family member 2 (SLAMF2); TCT.1

Summary

Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

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