Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human tonsil stained with Topo II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/6629).
Formalin-fixed, paraffin-embedded human lymph node stained with Topo II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/6629).
SDS-PAGE Analysis of Purified Topo IIa, Recombinant Mouse Monoclonal Antibody (rTOP2A/6629). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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4
1
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6
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It recognizes a 170kDa protein, which is identified as topoisomerase II伪. It shows no cross-reaction with Topoisomerase II 尾 or I. Topo II 伪 plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II 伪 is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
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