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Formalin-fixed, paraffin-embedded human Skin stained with S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2).
Formalin-fixed, paraffin-embedded human Placenta stained with S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2).
Immunofluorescence Analysis of A549 cells labeling S100A2 with S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Flow Cytometric Analysis of trypsinised HeLa cells. S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of A-431 lysate using S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2)
SDS-PAGE Analysis Purified S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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S100A2 belongs to the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100A2 may function as a modulator against excess calcium accumulation in normal human mammary epithelial cells and also have a role in suppressing tumor cell growth. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer. Cytoplasmic overexpression may also be of prognostic significance when observed in oral cancer patients, and the S100A2 has also been identified as significantly down-regulated in gastric cancer.
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