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Recombinant Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody [ACTA2/1614R]

Additional information

Catalog Number: 59-RBM4
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
59-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
59-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
59-RBM4-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody

Formalin-fixed, paraffin-embedded human uterus stained with Smooth Muscle Actin Recombinant Rabbit Monoclonal (ACTA2/1614R).

Recombinant Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Smooth Muscle Actin Recombinant Rabbit Monoclonal (ACTA2/1614R).

Recombinant Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified SMA Recombinant Rabbit Monoclonal Antibody (ACTA2/1614R). Confirmation of Integrity and Purity of Antibody.

Actin, Smooth Muscle (ACTA2) Antibody in Western Blot (WB)

Actin, Smooth Muscle (ACTA2) Antibody in Western Blot (WB)

Western Blot Analysis of Mouse 3T3 lysate using ACTA2 Rabbit Recombinant Monoclonal Antibody (ACTA2/1614R).

Actin, Smooth Muscle (ACTA2) Antibody in Western Blot (WB)

Actin, Smooth Muscle (ACTA2) Antibody in Western Blot (WB)

Western Blot Analysis of Mouse NIH/3T3 and human kidney tissue lysates using Actin, Smooth Muscle (ACTA2) Rabbit Recombinant Monoclonal Antibody (ACTA2/1614R).

ACTA2 Antibody in Western Blot (WB)

ACTA2 Antibody in Western Blot (WB)

Western Blot analysis of Human Kidney tissue lysate using ACTA2 Recombinant Rabbit Monoclonal Antibody (ACTA2/1614R)

Actin Antibody in Western Blot (WB)

Actin Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney and Rabbit Kidney tissue lysates using Actin Recombinant Rabbit Monoclonal Antibody (ACTA2/1614R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
ACTA2/1614R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Cytoskeleton
Gene Name
ACTA2
Positive Control
Kidney, skeletal muscle, Human uterus or leiomyosarcoma, Mouse NIH/3T3 cells.
Immunogen
Recombinant fragment corresponding to the n-terminal region of the human Smooth Muscle Actin protein
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
59
SwissProt
P62736
Chromosome Location
10q23.31
Mol. Weight of Antigen
42.01kDa
Alternate Names
Actin, aortic smooth muscle, ACTSA|SMDYS

Summary

Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

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