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Uroplakin 1B (Urothelial Differentiation Marker) Antibody [MSVA-734M]

Additional information

Catalog Number: 7348-MSM15
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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UPK1B Antibody in Numerous Tissues (IHC)

UPK1B Antibody in Numerous Tissues (IHC)

Uroplakin-1b Mouse Monoclonal Antibody (MSVA-734M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
In Stock
Catalog Number Formulation Availability Size Price
7348-MSM15-P0
Purified Ab with BSA and Azide
Trial Size
In Stock
20ug
$324.50
Trial Size Available In-Stock
7348-MSM15-P1
Purified Ab with BSA and Azide
In Stock
100ug
$599.50
In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-734M
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Membrane
Gene Name
UPK1B
Positive Control
Urinary bladder: A strong membranous and cytoplasmic UPK1b immunostaining should be seen in the urothelium (the staining can be limited to the top cell layers or equally involve all cell layers).
Immunogen
Recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B) protein (exact sequence is proprietary)
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab purified from Bioreactor Concentrate by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity).
Key References
  • Carpenter AR, et al. Kidney Int. 2016;89(3):612-24.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7348
SwissProt
O75841
Chromosome Location
3q13.32
Mol. Weight of Antigen
30kDa
Alternate Names
Uroplakin-1b, Tetraspanin-20, Uroplakin Ib, Tetraspanin-20; Tspan-20; TSPAN20; UP1b; UPIb; UPK1B; Uroplakin-1b

Summary

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.UPK1B is expressed by terminally differentiated urothelial cells.

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