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Uroplakin 1A (Urothelial Differentiation Marker) Antibody [MSVA-735M]

In Stock
Catalog Number Formulation Size Price
11045-MSM14-P0
Purified Ab with BSA and Azide
20ug
$324.50
11045-MSM14-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
UPK1A Antibody in Numerous Tissues (IHC)

UPK1A Antibody in Numerous Tissues (IHC)

Uroplakin-1a Mouse Monoclonal Antibody (MSVA-735M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Membrane
Gene Name
UPK1A
Positive Control
Urinary bladder: A strong membranous and cytoplasmic Upk1a immunostaining should be seen in the urothelium (the staining can be limited to the top 1/3 or 2/3 of the urothelium).
Immunogen
Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)
Alternate Names
Uroplakin-1a, Tetraspanin-21, Uroplakin Ia, UP1A; UPIA; UPKA; TSPAN21

Database Links

Entrez Gene ID
11045
SwissProt
O00322

Additional Information

Clone
MSVA-735M
Chromosome Location
19q13.12
Mol. Weight of Antigen
29kDa

Related Antibodies

View All UPK1A Antibodies

Functions

  • Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity).

Key References

  • Sun TT, Liang FX, Wu XR. Uroplakins as markers of urothelial differentiation. Adv Exp Med Biol. 1999;462:7-18; discussion 103-14.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab purified from Bioreactor Concentrate by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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