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SI-CLP / Chitinase Domain Containing 1 (CHID1) Antibody [CHID1/6368]

Additional information

Catalog Number: 66005-MSM1
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
66005-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
66005-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
66005-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
SI-CLP / Chitinase Domain Containing 1 (CHID1) Antibody

Formalin-fixed, paraffin-embedded human esophagus stained with SI-CLP / CHID1 Mouse Monoclonal Antibody (CHID1/6368) Inset: PBS instead of primary antibody; secondary only negative control.

SI-CLP / Chitinase Domain Containing 1 (CHID1) Antibody - Image 2

Formalin-fixed, paraffin-embedded human prostate stained with SI-CLP / CHID1 Mouse Monoclonal Antibody (CHID1/6368) HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
CHID1/6368
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Secreted. Lysosome.
Gene Name
CHID1
Positive Control
colon|HeLa cells. Human fetal gut|liver or heart.
Immunogen
Recombinant full-length human CHID1 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).
Key References
  • Kzhyshkowska, J., et al. 2006. Novel stabilin-1 interacting chitinase-like protein (SI-CLP) is up-regulated in alternatively activated macrophages and secreted via lysosomal pathway. Blood 107: 3221-3228.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
66005
SwissProt
Q9BWS9
Chromosome Location
11p15.5
Mol. Weight of Antigen
42-48kDa
Alternate Names
Chitinase domain-containing protein 1, Stabilin-1-interacting chitinase-like protein, CHID1; Chitinase domain containing 1; FLJ42707; GL008; MGC3234; PSEC0104; SB139; SI-CLP; SICLP; Stabilin 1 interacting chitinase like protein

Summary

SI-CLP (stabilin-1 interacting chitinase-like), also known as CHID1 (chitinase domain-containing protein 1), is a 393 amino acid secreted protein that belongs to the glycosyl hydrolase 18 family. SI-CLP interacts with STAB1 and localizes to lysosome in cells of monocytic, T, B and epithelial origin. SI-CLP is a saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro). The SI-CLP protein is up-regulated by IL4/interleukin-4 and dexamethasone in the macrophages, and is also up-regulated by glucocorticoid. Existing as three alternatively spliced isoforms, the SI-CLP gene is conserved in chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish, fruit fly, mosquito, A. thaliana and rice, and maps to human chromosome 11p15.5.

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