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SERPINB5 / Maspin (Tumor Suppressor & Epithelial Biomarker) Antibody [SERPINB5/12555]

In Stock
Catalog Number Formulation Size Price
5268-MSM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
5268-MSM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
5268-MSM12-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Summary

Maspin, a member of the serpin (serine protease inhibitor) superfamily encoded by the SERPINB5 gene, was originally identified in normal human mammary epithelial cells. Serpins are a protein family that regulate chymotrypsin-like serine proteases, playing roles in controlling activated proteases and regulating cell death. Structurally, Maspin is located in the extracellular matrix and at the plasma membrane, where it exerts its function at the cell surface by inhibiting cell motility and invasion, particularly in breast and prostate cancer cells.
Maspin is expressed in normal mammary and prostate epithelial cells but is often absent or downregulated in their corresponding tumor cells, despite no significant structural mutations in the Maspin gene being detected in these cancers. It acts as a tumor suppressor, particularly in epithelial cells, where it inhibits cancer cell invasion and metastasis. In primary breast cancer, Maspin expression is regulated by wild-type p53, linking it to a molecular mechanism that suppresses tumor invasion and metastasis. Loss of Maspin expression is associated with more aggressive tumors and worse prognosis in advanced breast and prostate cancers. Maspin expression varies significantly across cancers. While it is frequently downregulated in breast and prostate tumors, it is overexpressed in pancreatic, ovarian, thyroid, gastric, lung, bladder, skin, colon, and some breast cancers. In primary non-small cell lung cancer (NSCLC), general Maspin expression correlates with poorer overall survival, yet strong nuclear Maspin expression is linked to better overall and disease-free survival in patients with resectable NSCLC.
In summary, Maspin is a multifunctional protein with dual roles in cancer, acting as a tumor suppressor in some contexts while being overexpressed in others. Its expression patterns and subcellular localization, particularly nuclear versus cytoplasmic, have significant implications for tumor behavior and patient prognosis.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Cytoplasm, Extracellular space, Nucleus, Secreted
Gene Name
Positive Control
Squamous epithelium or gastrointestinal tissue.
Immunogen
Recombinant fragment (around aa1-200) of human SERPINB5 protein (exact sequence is proprietary)
Alternate Names
Serpin B5, Maspin, Peptidase inhibitor 5, Maspin; Ovalbumin; PI-5; PI5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin family B member 5; Serpin peptidase inhibitor, clade B (ovalbumin), member 5; Spi5; Spi7

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
SERPINB5/12555
Chromosome Location
18q21.33
Mol. Weight of Antigen
42kDa

Functions

  • Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Key References

  • Zou, Z., et al. 1994. Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells. Science 263: 526-529.
  • Zheng HC, et al. Cytoplasmic and nuclear maspin expression in lung carcinomas: an immunohistochemical study using tissue microarrays. Appl Immunohistochem Mol Morphol. 2008;16(5):459-465.
  • Märkl, Bruno et al. “Shift from cytoplasmic to nuclear maspin expression correlates with shorter overall survival in node-negative colorectal cancer.” Human pathology vol. 41,7 (2010): 1024-33. doi:10.1016/j.humpath.2009.10.021
  • Sopel, M et al. “Nuclear maspin expression as a good prognostic factor in human epithelial ovarian carcinoma.” Folia morphologica vol. 69,4 (2010): 204-12.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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