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Recombinant von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Antibody [VWF/7979R]

Additional information

Catalog Number: 7450-RBM20
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7450-RBM20-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7450-RBM20-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7450-RBM20-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Antibody

Formalin-fixed, paraffin-embedded human uterus stained with vWF Recombinant Rabbit Monoclonal Antibody (VWF/7979R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified vWF Recombinant Rabbit Monoclonal Antibody (VWF/7979R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
VWF/7979R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Extracellular matrix, Extracellular space, Secreted
Gene Name
VWF
Positive Control
Human tonsil.
Immunogen
Recombinant fragment (around aa1208-1222) of the human von Willebrand Factor (vWF) protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7450
SwissProt
P04275
Chromosome Location
12p13.31
Mol. Weight of Antigen
309.26kDa
Alternate Names
von Willebrand factor, F8VWF|VWD

Summary

Human von Willebrand factor (VWF or factor VIII-related antigen) is a 309 kDa multimeric plasma glycoprotein, important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Von Willebrand factor also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Von Willebrand factor is synthesized by endothelial cells and is reported to be expressed in a number of tumors of vascular origin. IHC results aid in the classification of acute myeloid leukemia FAB type M7, angiosarcoma and epithelioid hemangioendothelioma.

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