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Recombinant VEGF (Vascular Endothelial Growth Factor) Antibody [VEGFA/7757R]

Additional information

Catalog Number: 7422-RBM7
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7422-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7422-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7422-RBM7-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant VEGF (Vascular Endothelial Growth Factor) Antibody

Formalin-fixed, paraffin-embedded human placenta stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7757R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant VEGF (Vascular Endothelial Growth Factor) Antibody - Image 2

Formalin-fixed, paraffin-embedded human placenta stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7757R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

VEGFA Antibody in Western Blot (WB)

VEGFA Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney and Human Lung tissue lysates using VEGFA Recombinant Rabbit Monoclonal Antibody (VEGFA/7757R).

VEGFA Antibody in Western Blot (WB)

VEGFA Antibody in Western Blot (WB)

Western Blot Analysis of Lung tissue lysates of different species using VEGFA Recombinant Rabbit Monoclonal Antibody (VEGFA/7757R).

VEGFA Antibody in SDS-PAGE

VEGFA Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Vascular endothelial growth factor A, long form | N-VEGF | VEGFA Recombinant Rabbit Monoclonal Antibody (VEGFA/7757R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
VEGFA/7757R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Endoplasmic reticulum, Extracellular matrix, Extracellular space, Golgi apparatus, Nucleus, Secreted
Gene Name
VEGFA
Positive Control
breast or ovarian carcinomas.|Tumor cells in astrocytomas, Kidney, Lung
Immunogen
Recombinant fragment (around aa64-77) of human VEGFA protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity)., Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed:34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437)., Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7422
SwissProt
P15692
Chromosome Location
6p21.1
Mol. Weight of Antigen
43.6kDa
Alternate Names
Vascular endothelial growth factor A, long form, L-VEGF|MVCD1|VEGF|VPF

Summary

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

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