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Recombinant TRIM29 (Lung Squamous Cell Carcinoma Marker) Antibody [TRIM29/9256R]

Additional information

Catalog Number: 23650-RBM6
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
23650-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
23650-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
23650-RBM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant TRIM29 (Lung Squamous Cell Carcinoma Marker) Antibody

Formalin-fixed, paraffin-embedded human esophagus stained with TRIM29 Recombinant Rabbit Monoclonal Antibody (TRIM29/9256R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant TRIM29 (Lung Squamous Cell Carcinoma Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified TRIM29 Recombinant Rabbit Monoclonal Antibody (TRIM29/9256R). Confirmation of Purity and Integrity of Antibody.

TRIM29 Antibody in Western Blot (WB)

TRIM29 Antibody in Western Blot (WB)

Western Blot analysis of A431 cell lysate using TRIM29 Recombinant Rabbit Monoclonal Antibody (TRIM29/9256R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
TRIM29/9256R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Lysosome
Gene Name
TRIM29
Positive Control
A431 cells. Human tonsil or squamous cell carcinoma.
Immunogen
Recombinant fragment (around aa4-129) of the human TRIM29 protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
23650
SwissProt
Q14134
Chromosome Location
11q23.3
Mol. Weight of Antigen
65.83kDa
Alternate Names
Tripartite motif-containing protein 29, ATDC

Summary

It recognizes a 66kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29). It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90% positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

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