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Recombinant Thrombomodulin / CD141 (Endothelial Cell Marker) Antibody [MSVA-141M]

In Stock
Catalog Number Formulation Size Price
7056-MSM6-P0
Purified Ab with BSA and Azide
20ug
$324.50
7056-MSM6-P1
Purified Ab with BSA and Azide
100ug
$654.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
THBD Antibody in Numerous Tissues (IHC)

THBD Antibody in Numerous Tissues (IHC)

Thrombomodulin Mouse Recombinant Monoclonal Antibody (MSVA-141M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi's sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Endothelial Cell Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane
Gene Name
THBD
Positive Control
Lung: A strong staining should be seen in endothelial cells, and at least a weak to moderate membranous CD141 staining should be seen in alveolar macrophages.
Immunogen
Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein (exact sequence is proprietary)
Alternate Names
Thrombomodulin, Fetomodulin, AHUS6; BDCA3; CD141; Fetomodulin; Thbd; THPH12; THRM; Thrombomodulin (TM)

Database Links

Entrez Gene ID
7056
SwissProt
P07204

Additional Information

Clone
MSVA-141M
Chromosome Location
20p11.2
Mol. Weight of Antigen
100kDa

Related Antibodies

View All THBD Antibodies

Functions

  • Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

Key References

  • Appleton MA, Attanoos RL, Jasani B. Thrombomodulin as a marker of vascular and lymphatic tumours. Histopathology. 1996 Aug;29(2):153-7.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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