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Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody [SPTA1/2939R]

Additional information

Catalog Number: 6708-RBM5
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6708-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6708-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6708-RBM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin alpha 1 Rabbit Recombinant Monoclonal Antibody (SPTA1/2939R).

Recombinant Spectrin Alpha 1 (Erythrocyte Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin alpha 1 Rabbit Recombinant Monoclonal Antibody (SPTA1/2939R).

Spectrin alpha, erythrocytic 1 Antibody in Western Blot (WB)

Spectrin alpha, erythrocytic 1 Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney and Human Lung tissue lysates using Spectrin alpha, erythrocytic 1 Recombinant Rabbit Monoclonal Antibody (SPTA1/2939R).

Spectrin alpha, erythrocytic 1 Antibody in Western Blot (WB)

Spectrin alpha, erythrocytic 1 Antibody in Western Blot (WB)

Western Blot Analysis of Kidney and Lung tissue lysates of different species using Spectrin alpha, erythrocytic 1 Recombinant Rabbit Monoclonal Antibody (SPTA1/2939R).

SPTA1 Antibody in SDS-PAGE

SPTA1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Spectrin alpha chain, erythrocytic 1 Rabbit Monoclonal Antibody (SPTA1/2939R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
SPTA1/2939R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell cortex, Cytoplasm, Cytoskeleton
Gene Name
SPTA1
Positive Control
HeLa, Jurkat cells. Placenta or Tonsil., K562, Human Kidney, Human Lung
Immunogen
Recombinant fragment (around aa356-475) of the human Spectrin alpha, erythrocytic 1 (SPTA1) protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6708
SwissProt
P02549
Chromosome Location
1q23.1
Mol. Weight of Antigen
280.01kDa
Alternate Names
Spectrin alpha chain, erythrocytic 1, EL2|HPP|HS3|SPH3|SPTA

Summary

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

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