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Recombinant Occludin (Marker of Early Blood Brain Barrier Damage) Antibody [rOCLN/8776]

In Stock
Catalog Number Formulation Size Price
100506658-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
100506658-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
100506658-MSM11-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Occludin (Marker of Early Blood Brain Barrier Damage) Antibody

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Occludin (Marker of Early Blood Brain Barrier Damage) Antibody - Image 2

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Occludin (Marker of Early Blood Brain Barrier Damage) Antibody - Image 3

SDS-PAGE Analysis of Purified Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776) Confirmation of Purity and Integrity of Antibody.

Occludin Antibody in Western Blot (WB)

Occludin Antibody in Western Blot (WB)

Western Blot Analysis of human kidney tissue lysate using Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions., (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell junction, Cell membrane, Tight junction
Gene Name
OCLN
Positive Control
Localized at tight junctions of both epithelial and endothelial cells
Immunogen
Recombinant fragment (around aa282-415) of the human Occludin protein (exact sequence is proprietary)
Alternate Names
Occludin, BLCPMG|PPP1R115|PTORCH1

Database Links

Entrez Gene ID
100506658
SwissProt
Q16625

Additional Information

Clone
rOCLN/8776
Chromosome Location
5q13.2
Mol. Weight of Antigen
59.14kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All OCLN Antibodies

Functions

  • May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions., (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

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