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Recombinant HLA-DP (MHC II) Antibody [HLA-Pan/9268R]

Additional information

Catalog Number: RBM8-9268
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
RBM8-9268-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
RBM8-9268-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
RBM8-9268-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant HLA-DP (MHC II) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Pan Rabbit Recombinant Monoclonal Antibody (HLA-Pan/9268R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant HLA-DP (MHC II) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Pan Rabbit Recombinant Monoclonal Antibody (HLA-Pan/9268R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant HLA-DP (MHC II) Antibody - Image 3

SDS-PAGE Analysis of Purified HLA-Pan Rabbit Recombinant Monoclonal (HLA-Pan/9268R). Confirmation of Purity and Integrity of Antibody.

Pan-HLA-II (DP, DQ, DR) Antibody in Western Blot (WB)

Pan-HLA-II (DP, DQ, DR) Antibody in Western Blot (WB)

Western blot analysis of Daudi cell lysate using Pan-HLA-II (DP, DQ, DR) Recombinant Rabbit Monoclonal Antibody (HLA-Pan/9268R).

Pan-HLA-II (DP, DQ, DR) Antibody in Western Blot (WB)

Pan-HLA-II (DP, DQ, DR) Antibody in Western Blot (WB)

Western blot analysis of Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine, Human Lung, Mouse Lung, Rat Lung, Hamster Lung and Guinea pig Lung tissue lysates using Pan-HLA-II (DP, DQ, DR) Recombinant Rabbit Monoclonal Antibody (HLA-Pan/9268R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
HLA-Pan/9268R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, trans-Golgi network membrane
Gene Name
HLA-DPB1
Positive Control
Daudi, Ramos or Raji cells. Human tonsil or lymph node. Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine, Human Lung, Mouse Lung, Rat Lung, Hamster Lung or Guinea pig Lung
Immunogen
Recombinant fragment (around aa190-204) of the human Pan-HLA-II (DP, DQ, DR) protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
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Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3115
SwissProt
P04440
Chromosome Location
6p21.32
Mol. Weight of Antigen
29.16kDa
Alternate Names
HLA class II histocompatibility antigen, DP beta 1 chain, DPB1|HLA-DP|HLA-DP1B|HLA-DPB

Summary

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.

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