Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant Glucagon (Marker of Glucagonoma) Antibody [GCG/13090R]

In Stock
Catalog Number Formulation Size Price
2641-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
2641-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
2641-RBM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
How do I understand the HuProtTM Validation Results? Learn More
Glucagon Antibody in Immunohistochemistry (IHC (P))

Glucagon Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human pancreas stained with Glucagon Recombinant Rabbit Monoclonal Antibody (GCG/13090R). Inset: PBS instead of primary antibody; secondary only negative control.

Glucagon Antibody in SDS-PAGE

Glucagon Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Glucagon Recombinant Rabbit Monoclonal Antibody (GCG/13090R). Confirmation of Purity and Integrity of Antibody.

Glucagon Antibody in Human Protein Array (HuProt) Analysis

Glucagon Antibody in Human Protein Array (HuProt) Analysis

Analysis of Protein Array containing more than 19,000 full-length human proteins using Glucagon Recombinant Rabbit Monoclonal Antibody (GCG/13090R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Glucagon is a pancreatic hormone that functions as an antagonist to Insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagon-like peptide-1 (GLP-1), Glucagon-like peptide-2 (GLP-2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the Glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increase the synthesis of several hormones, including growth hormone. Glucagon antibody detects glucagon-secreting cells and tumors such as glucagonomas, of which approximately 80% are malignant.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Endocrine, Gastrointestinal Tract, Metabolism, Digestion, Lipid Metabolism
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Secreted
Gene Name
GCG
Positive Control
Human pancreas.
Immunogen
Recombinant fragment (around aa1-100) of human GCG protein (exact sequence is proprietary)
Alternate Names
Pro-glucagon, Glicentin; Glicentin-related polypeptide (GRPP); Oxyntomodulin (OXM; OXY); Glucagon; Glucagon-like peptide 1 (GLP-1); Alternative names: Incretin hormone Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2), GLP1; GLP2; GRPP; GLP-1

Database Links

Entrez Gene ID
2641
SwissProt
P01275

Additional Information

Clone
GCG/13090R
Chromosome Location
2q24.2
Mol. Weight of Antigen
19kDa (Proglucagon); 3kDa (Glucagon)

Related Antibodies

View All GCG Antibodies

Functions

  • Glucagon Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon-like peptide 1 Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed:22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable). Glucagon-like peptide 2 Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. Oxyntomodulin Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness. Glicentin May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.

Key References

  • Kolb et al (2009). Glucagon/insulin ratio as a potential biomarker for pancreatic cancer in patients with new-onset diabetes mellitus. Cancer biology & therapy, 8(16), 1527–1533. https://doi.org/10.4161/cbt.8.16.9006
  • Janah, L., Kjeldsen, S., et al. (2019). Glucagon Receptor Signaling and Glucagon Resistance. International journal of molecular sciences, 20(13), 3314. https://doi.org/10.3390/ijms20133314
  • Thakkar, A., Wang, B. et al. (2016). Vitamin D and androgen receptor-targeted therapy for triple-negative breast cancer. Breast cancer research and treatment, 157(1), 77–90. https://doi.org/10.1007/s10549-016-3807-y
  • Capozzi, M. E., D'Alessio, D. A., & Campbell, J. E. (2022). The past, present, and future physiology and pharmacology of glucagon. Cell metabolism, 34(11), 1654–1674. https://doi.org/10.1016/j.cmet.2022.10.001

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Glucagon (Marker of Glucagonoma) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales