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Recombinant Galectin-3 (LGALS3) (Thyroid Cell Marker) Antibody [rLGALS3/7286]

Additional information

Catalog Number: 3958-MSM6
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3958-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3958-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3958-MSM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Galectin-3 (LGALS3) (Thyroid Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human colon stained with Galectin-3 Recombinant Mouse Monoclonal Antibody (rLGALS3/7286). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Galectin-3 Antibody in Western Blot (WB)

Galectin-3 Antibody in Western Blot (WB)

Western Blot Analysis of MCF-7 lysate using Galectin-3 Mouse Recombinant Monoclonal Antibody (rLGALS3/7286)

LGALS3 Antibody in SDS-PAGE

LGALS3 Antibody in SDS-PAGE

SDS-PAGE Analysis Purifie LGALS3 Recombinant Mouse Monoclonal Antibody (rLGALS3/7286). Confirmation of Purity and Integrity of Antibody.

Galectin-3 Antibody in Western Blot (WB)

Galectin-3 Antibody in Western Blot (WB)

Western Blot Analysis of Human Heart, Human Lung, Human Skin,A431 and PANC28 lysates using LGALS3 Recombinant Mouse Monoclonal Antibody (rLGALS3/7286).

Galectin-3 Antibody in Western Blot (WB)

Galectin-3 Antibody in Western Blot (WB)

Western Blot Analysis of Heart, Lung and Skin tissue lysates of different species using LGALS3 Recombinant Mouse Monoclonal Antibody (rLGALS3/7286).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rLGALS3/7286
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus, Secreted
Gene Name
LGALS3
Positive Control
Human papillary thyroid carcinoma tissue, MCF-7, Heart, Lung, Skin, A431 or PANC28.
Immunogen
Recombinant fragment (around aa163-191) of the human Galectin-3 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3958
SwissProt
P17931
Chromosome Location
14q22.3
Mol. Weight of Antigen
26.15kDa
Alternate Names
Galectin-3, CBP35|GAL3|GALBP|GALIG|L31|LGALS2|MAC2

Summary

Galectin-3 is a member of the beta-galactosidase-binding lectin family. It is associated with cell growth, adhesion, inflammation, mRNA processing, and apoptosis. Aberrant expression of Galectin-3 is related to malignant transformation and metastasis in carcinomas of the breast, colon and thyroid.Galectin-3 reactivity can be seen in the nucleus of neutrophils, vascular endothelium, carcinomas of the colon, breast, and thyroid. Galectin-3 may be useful in the differentiation �of benign and malignant thyroid neoplasms.Galectin-3 may also be useful in the identification of certain liver disorders.

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