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Recombinant Estrogen Receptor (Marker of Estrogen Dependence) Antibody [rESR1/9525]

Additional information

Catalog Number: 2099-MSM51
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2099-MSM51-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2099-MSM51-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2099-MSM51-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Estrogen Receptor alpha Antibody in Western Blot (WB)

Estrogen Receptor alpha Antibody in Western Blot (WB)

Western Blot Analysis of MCF7 lysate using Estrogen Receptor alpha Recombinant Mouse Monoclonal Antibody (rESR1/9525).

ESR1 Antibody in SDS-PAGE

ESR1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Estrogen Receptor alpha Recombinant Mouse Monoclonal Antibody (rESR1/9525). Confirmation of Purity and Integrity of Antibody.

ESR1 Antibody in Immunohistochemistry (IHC (P))

ESR1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human breast stained with Estrogen Receptor alpha Recombinant Mouse Monoclonal Antibody (rESR1/9525). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Estrogen Receptor alpha Antibody in Western Blot (WB)

Estrogen Receptor alpha Antibody in Western Blot (WB)

Western Blot Analysis of HeLa and MCF7 cell lysates using Estrogen Receptor alpha Recombinant Mouse Monoclonal Antibody (rESR1/9525).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rESR1/9525
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cell membrane, Cytoplasm, Golgi apparatus, Nucleus
Gene Name
ESR1
Positive Control
HeLa, MCF7 cells. Human breast or endometrial carcinoma.
Immunogen
Recombinant fragment (around aa578-595) of the human Estrogen Receptor protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity)., Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2099
SwissProt
P03372
Chromosome Location
6q25.1-q25.2
Mol. Weight of Antigen
66.22kDa
Alternate Names
Estrogen receptor, ER|ESR|ESRA|ESTRR|Era|NR3A1

Summary

Recognizes a protein of 67kDa, which is identified as estrogen receptor (ER) alpha. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas.The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.

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