Printable Datasheet

Recombinant EPH Receptor B4 (EPHB4) Antibody [EPHB4/9663R]

In Stock
Catalog Number Formulation Size Price
2050-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2050-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2050-RBM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
EPHB4 Antibody in Immunohistochemistry (IHC (P))

EPHB4 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed human liver stained with EPHB4 Recombinant Rabbit Monoclonal Antibody (EPHB4/9663R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

EPHB4 Antibody in SDS-PAGE.

EPHB4 Antibody in SDS-PAGE.

SDS-PAGE Analysis of EPHB4 Recombinant Rabbit Monoclonal Antibody (EPHB4/9663R). Confirmation of Purity and Integrity of Antibody.

EPH Receptor B4 (EPHB4) Antibody in Western Blot (WB)

EPH Receptor B4 (EPHB4) Antibody in Western Blot (WB)

Western blot analysis of Human Lung, Human Skin and Human Spleen tissue lysates using EPHB4 Recombinant Rabbit Monoclonal Antibody (EPHB4/9663R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date. While the biological activities of these receptors have yet to be determined, there is increasing evidence that they are involved in central nervous system function and in development. The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1 (Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8 (Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3 (Cek10, Hek2), EphB4 (Htk), EphB5 (Hek9) and EphB6 (Mep). Ligands for Eph receptors include ephrin-A4 (LERK-4) which binds EphA3 and EphB1. In addition, ephrin-A2 (ELF-1) has been described as the ligand for EphA4, ephrin-A3 (Ehk1-L) as the ligand for EphA5 and ephrin-B2 (Htk-L) as the ligand for EphB4 (Htk).

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
EPHB4
Positive Control
Human tonsil, hepatocellular carcinoma or histiocytoma. Lung, Skin, Spleen
Immunogen
Recombinant fragment (around aa25-150) of the human EPH Receptor B4 (EPHB4) protein (exact sequence is proprietary)
Alternate Names
Ephrin type-B receptor 4, CMAVM2|HFASD|HTK|LMPHM7|MYK1|TYRO11

Database Links

Entrez Gene ID
2050
SwissProt
P54760

Additional Information

Clone
EPHB4/9663R
Chromosome Location
7q22.1
Mol. Weight of Antigen
108.27kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All EPHB4 Antibodies

Functions

  • Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales