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Recombinant EpCAM / CD326 (Epithelial Marker) Antibody [EGP40/7025R]

In Stock
Catalog Number Formulation Size Price
4072-RBM31-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4072-RBM31-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4072-RBM31-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant EpCAM / CD326 (Epithelial Marker) Antibody

SDS-PAGE Analysis Purified EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/7025R). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell junction, Lateral cell membrane, Tight junction
Gene Name
EPCAM
Positive Control
HT29 cells or breast tumor, MCF-7
Immunogen
Recombinant full-length human EpCAM protein
Alternate Names
Epithelial cell adhesion molecule, Ber-Ep4|BerEp4|DIAR5|EGP-2|EGP314|EGP40|ESA|HNPCC8|KS1/4|KSA|LYNCH8|M4S1|MIC18|MK-1|MOC-31|TACSTD1|TROP1

Database Links

Entrez Gene ID
4072
SwissProt
P16422

Additional Information

Clone
EGP40/7025R
Chromosome Location
2p21
Mol. Weight of Antigen
34.93kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All EPCAM Antibodies

Functions

  • May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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