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Recombinant Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody [rKRT5/6398]

Additional information

Catalog Number: 3852-MSM7
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3852-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3852-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3852-MSM7-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 5 Recombinant Mouse Monoclonal Antibody (rKRT5/6398).

Recombinant Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Cytokeratin 5 Recombinant Mouse Monoclonal Antibody (rKRT5/6398).

Recombinant Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody - Image 3

IHC analysis of formalin-fixed, paraffin-embedded human testis. Negative tissue control using rKRT5/6398 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker) Antibody - Image 4

SDS-PAGE Analysis of Purified KRT5 Recombinant Mouse Monoclonal Antibody (rKRT5/6398). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rKRT5/6398
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT5
Positive Control
esophagus or bladder tissue (IHC)., Human tonsil
Immunogen
Recombinant fragment corresponding to the c-terminal region of the human Cytokeratin 5 protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3852
SwissProt
P13647
Chromosome Location
12q13.13
Mol. Weight of Antigen
62.38kDa
Alternate Names
Keratin, type II cytoskeletal 5, CK5|DDD|DDD1|EBS1|EBS2|EBS2A|EBS2B|EBS2C|EBS2D|EBS2E|EBS2F|K5|KRT5A

Summary

This Mab recognizes a protein of 58kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, yoepithelial, and mesothelium.Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.

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