Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant Cytokeratin 18 (KRT18) Antibody [MSVA-118R]

In Stock
Catalog Number Formulation Size Price
3875-RBM17-P0
Purified Ab with BSA and Azide
20ug
$249.00
3875-RBM17-P1
Purified Ab with BSA and Azide
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
KRT18 Antibody in Numerous Tissues (IHC)

KRT18 Antibody in Numerous Tissues (IHC)

Keratin, type I cytoskeletal 18 Rabbit Recombinant Monoclonal Antibody (MSVA-118R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Nucleolus, Nucleus, Perinuclear region
Gene Name
KRT18
Positive Control
Liver: At least a weak to moderate, predominantly membranous staining should be seen in virtually all hepatocytes. Appendix: A strong staining should be seen in all epithelial cells.
Immunogen
Recombinant human full-length KRT18 protein
Alternate Names
Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

Database Links

Entrez Gene ID
3875
SwissProt
P05783

Additional Information

Clone
MSVA-118R
Chromosome Location
12q13.13
Mol. Weight of Antigen
45kDa

Related Antibodies

View All KRT18 Antibodies

Functions

  • Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Key References

  • Lauerova et al. Hybridoma7: 495-504 (1988).
  • Kovaik et al. Int.J.CancerSuppl.3: 50-5 (1988).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales