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Recombinant Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker) Antibody [KRT15/9088R]

Additional information

Catalog Number: 3866-RBM15
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3866-RBM15-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3866-RBM15-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3866-RBM15-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker) Antibody

SDS-PAGE Analysis of Purified KRT15 Rabbit Recombinant Monoclonal Antibody (KRT15/9088R). Confirmation of Purity and Integrity of Antibody.

Recombinant Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human prostate cancer stained with Cytokeratin 15 Rabbit Recombinant Monoclonal Antibody (KRT15/9088R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin 15 Rabbit Recombinant Monoclonal Antibody (KRT15/9088R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
KRT15/9088R
Isotype
IgG
Light Chain
Kappa
Gene Name
KRT15
Positive Control
A431 cells. Human skin or colon.|HeLa
Immunogen
Recombinant full-length human Cytokeratin 15 protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3866
SwissProt
P19012
Chromosome Location
17q21.2
Mol. Weight of Antigen
49.21kDa
Alternate Names
Keratin, type I cytoskeletal 15, CK15|K15|K1CO

Summary

Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. KRT15 is downregulated in activated keratinocytes. It is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

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