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Formalin-fixed, paraffin-embedded human placenta stained with Cyclin E Recombinant Rabbit Mouse Antibody (r13A3). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified Cyclin E Recombinant Mouse Monoclonal Antibody (r13A3). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Cyclin E was identified as a protein which would complement cyclin mutations in yeast and mammalian cells. Overexpression of cyclin E shortens the length of the G1 phase, accelerating progression of the cell cycle into S phase. The activity of cyclin E is mediated through its activation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of tumor suppressor proteins such as p16.
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