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Recombinant Collagen IV alpha 3 chain (COL4A3) Antibody [rCOL4A3/9539]

In Stock
Catalog Number Formulation Size Price
1285-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1285-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1285-MSM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
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Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a ‘chicken-wire’ meshwork together with laminins, proteoglycans and entactin/nidogen., Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
COL4A3
Positive Control
Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung
Immunogen
Recombinant fragment (around aa1445-1669) of the human Collagen IV alpha 3 chain protein (exact sequence is proprietary)
Alternate Names
Collagen alpha-3(IV) chain, ATS2|ATS3|ATS3A|ATS3B|BFH2

Database Links

Entrez Gene ID
1285
SwissProt
Q01955

Additional Information

Clone
rCOL4A3/9539
Chromosome Location
2q36.3
Mol. Weight of Antigen
161.81kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All COL4A3 Antibodies

Functions

  • Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen., Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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