Contact Us

Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody [rC86/6872]

Additional information

Catalog Number: 942-MSM22
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
In Stock
Catalog Number Formulation Size Price
942-MSM22-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
942-MSM22-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
942-MSM22-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody

IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Staining of cell surface using rC86/6872 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CD86 Rabbit Recombinant Monoclonal Antibody (rC86/6872). Confirmation of Integrity and Purity of Antibody.

CD86 Antibody in Western Blot (WB)

CD86 Antibody in Western Blot (WB)

Western Blot Analysis of Raji cell lysate using CD86 Recombinant Mouse Monoclonal Antibody (rC86/6872).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rC86/6872
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
CD86
Positive Control
293T or Jurkat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.
Immunogen
Recombinant fragment (around aa66-195) of the human CD86 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Western blot analysis of human heart tissue lysate using N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/6857R).
3
  • IHC
  • WB

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-RBM7
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).
4
  • IHC
  • WB

Occludin (Marker of Early Blood Brain Barrier Damage) Antibody

Additional information

Catalog Number: 100506658-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded Mouse Heart stained with N-Cadherin Mouse Monoclonal Antibody (CDH2/1573).
1
  • IHC

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM3
Reactivity:

,

Gene:

Host:

Flow cytometric analysis of PFA-fixed HeLa cells. MEF2B Mouse Monoclonal Antibody (PCRP-MEF2B-2F9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
2
  • Flow
  • IF
  • WB

MEF2B (Transcriptional Activator) Antibody

Additional information

Catalog Number: 100271849-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Cadherin 16 Mouse Monoclonal Antibody (CDH16/2448).
3
  • ELISA
  • IHC

Ksp-Cadherin / CDH16 (Renal Cell Marker) Antibody

Additional information

Catalog Number: 1014-MSM6
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2618).
4
  • Flow
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM8
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2616).
3
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM6
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH2/1426).
2
  • IHC
  • WB

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM4
Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity)., Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation., (Microbial infection) Acts as a receptor for adenovirus subgroup B.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
942
SwissProt
P42081
Chromosome Location
3q13.33
Mol. Weight of Antigen
37.68kDa
Alternate Names
T-lymphocyte activation antigen CD86, B7-2|B7.2|B70|CD28LG2|LAB72

Summary

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin’s disease.

Worry-Free Guarantee

Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

Learn More

Need Technical Help?

Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.

CONTACT US

Follow Us on LinkedIn

NeoBiotechnologies develops and manufactures highly specific, validated monoclonal antibodies for cancer research and diagnostics.

Phone

Whatsapp

Quick Links

Information

© 2026 NeoBiotechnologies.  All Rights Reserved.

Terms and Conditions Privacy Policy
Contact Us