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Recombinant CD44 / HCAM (Cancer Stem Cell Marker) Antibody [HCAM/2875R]

Additional information

Catalog Number: 960-RBM18
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
960-RBM18-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
960-RBM18-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
960-RBM18-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD44 / HCAM (Cancer Stem Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Lymph Node stained with CD44 Rabbit Recombinant Monoclonal Antibody (HCAM/2875R).

CD44 Antibody in Western Blot (WB)

CD44 Antibody in Western Blot (WB)

Western Blot Analysis of MDA-MB-231 lysate using CD44 Rabbit Recombinant Monoclonal Antibody (1HCAM/2875R)

CD44 Antibody in Western Blot (WB)

CD44 Antibody in Western Blot (WB)

Western Blot Analysis of MCF7 lysate using CD44 Rabbit Recombinant Monoclonal Antibody (HCAM/2875R)

CD44 Antibody in Western Blot (WB)

CD44 Antibody in Western Blot (WB)

Western Blot Analysis of Human Lung, Colon, Small Intestine, Tonsil tissue lysate using CD44 Recombinant Rabbit Monoclonal Antibody (HCAM/2875R).

CD44 Antibody in Western Blot (WB)

CD44 Antibody in Western Blot (WB)

Western Blot Analysis of Human Lung, Human Pancreas, A549, and HeLa lysates using CD44 Recombinant Rabbit Monoclonal Antibody (HCAM/2875R).

CD44 Antibody in SDS-PAGE

CD44 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD44 antigen Recombinant Rabbit Monoclonal Antibody (HCAM/2875R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Flow
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Clone
HCAM/2875R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane, Cell projection, Microvillus, Secreted
Gene Name
CD44
Positive Control
MCF7, HeLa or MDA-MB-231 cells. Paracortex in lymph node, MCF7, HeLa or MDA-MB-231 cells. Paracortex in lymph node, human lung, small intestine or tonsil.
Immunogen
Recombinant fragment (around aa1-742) of the human CD44 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Formalin-fixed, paraffin-embedded human Kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2183).
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Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4443). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
5
  • IHC
  • WB

MICA Antibody

Additional information

Catalog Number: 100507436-MSM3
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
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Reactivity:

, , , ,

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  • IHC
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Additional information

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Reactivity:

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Host:

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH2/1426).
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Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
960
SwissProt
P16070
Chromosome Location
11p13
Mol. Weight of Antigen
81.54kDa
Alternate Names
CD44 antigen, CDW44|CSPG8|ECM-III|ECMR-III|H-CAM|HCELL|HUTCH-1|HUTCH-I|Hermes-1|LHR|MC56|MDU2|MDU3|MIC4|Pgp1

Summary

Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

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