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Recombinant Bax (Apoptosis Marker) Antibody [BAX/13724R]

Additional information

Catalog Number: 581-RBM10
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
581-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
581-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
581-RBM10-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Bax (Apoptosis Marker) Antibody in Western Blot (WB)

Bax (Apoptosis Marker) Antibody in Western Blot (WB)

Western Blot Analysis of Human Intestine, Human Kidney, Human Lung, Human Spleen, Human Stomach, A549 and MCF7 lysates using Bax (Apoptosis Marker) Recombinant Rabbit Monoclonal Antibody (BAX/13724R).

Bax (Apoptosis Marker) Antibody in Western Blot (WB)

Bax (Apoptosis Marker) Antibody in Western Blot (WB)

Western Blot Analysis of Lung, Kidney, Spleen and Stomach tissue lysates of different species using Bax (Apoptosis Marker) Recombinant Rabbit Monoclonal Antibody (BAX/13724R).

BAX Antibody in SDS-PAGE

BAX Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified BAX Recombinant Rabbit Monoclonal Antibody (BAX/13724R).Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
BAX/13724R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Mitochondrion outer membrane, Nucleus
Gene Name
BAX
Positive Control
A549, MCF7, HL-60, Jurkat, K562 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma, Intestine, Lung, Kidney, Spleen, A549, MCF7, Stomach
Immunogen
Recombinant fragment (around aa1-100) of the human BAX protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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5
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MICA Antibody

Additional information

Catalog Number: 100507436-MSM3
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
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Host:

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Other Information

Functions
  • Plays a role in the mitochondrial apoptotic process (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:36361894, PubMed:8358790, PubMed:8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
581
SwissProt
Q07812
Chromosome Location
19q13.33
Mol. Weight of Antigen
21.18kDa
Alternate Names
Apoptosis regulator BAX, BCL2L4

Summary

Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

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