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PRAME nuclear receptor transcriptional regulator Antibody [PRAME/9188]

Additional information

Catalog Number: 23532-MSM8
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
23532-MSM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
23532-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
23532-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
PRAME nuclear receptor transcriptional regulator Antibody

Formalin-fixed, paraffin-embedded human melanoma stained with PRAME Mouse Monoclonal Antibody (PRAME/9188). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

PRAME nuclear receptor transcriptional regulator Antibody - Image 2

SDS-PAGE Analysis of Purified PRAME Mouse Monoclonal Antibody (PRAME/9188). Confirmation of Purity and Integrity of Antibody.

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

Western blot analysis of human lung lysate using PRAME nuclear receptor transcriptional regulator Mouse Monoclonal Antibody (PRAME/9188).

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

PRAME nuclear receptor transcriptional regulator Antibody in Western Blot (WB)

Western blot analysis of SK-MEL-2 cell lysate using PRAME nuclear receptor transcriptional regulator Mouse Monoclonal Antibody (PRAME/9188).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
PRAME/9188
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cell membrane, Chromosome, Cytoplasm, Golgi apparatus, Nucleus
Gene Name
PRAME
Positive Control
SK-MEL-2 cells. Human testis or melanoma. lung.
Immunogen
Recombinant fragment (around aa100-250) of human PRAME protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation (PubMed:21822215, PubMed:26138980). The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding (PubMed:26138980). In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation (PubMed:21822215). Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis (PubMed:16179254).
Key References
  • Ikeda H, Lethe B, Lehmann F, et al. Characterization of an antigen that is recognized on a melanoma showing partial HLA loss by CTL expressing an NK inhibitory receptor. Immunity. 1997;6(2):199-208. doi:10.1016/s1074-7613(00)80426-4
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
23532
SwissProt
P78395
Chromosome Location
22q11.22
Mol. Weight of Antigen
58kDa
Alternate Names
Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, Preferentially expressed antigen of melanoma, Cancer/testis antigen 130; CT130; MAPE; Melanoma antigen preferentially expressed in tumors; OIP 4; OIP-4; OIP4; OPA interacting protein 4; Opa interacting protein OIP4; OPA-interacting protein 4; PRAME; Preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma; RP23-250F8.3

Summary

Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

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