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Phospho C-Jun (Thr91, Thr93) Antibody [C-J 4C4/1]

In Stock
Catalog Number Formulation Size Price
3725-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
3725-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
3725-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Phospho C-Jun (Thr91, Thr93) Antibody

Formalin-fixed, paraffin-embedded human Cervical Tumor stained with Phospho c-Jun Mouse Monoclonal Antibody (C-J 4C4/1).

Phospho C-Jun (Thr91, Thr93) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Cervical Tumor stained with Phospho c-Jun Mouse Monoclonal Antibody (C-J 4C4/1).

Phospho C-Jun (Thr91, Thr93) Antibody - Image 3

SDS-PAGE Analysis of Purified Phospho c-Jun Mouse Monoclonal Antibody (C-J 4C4/1). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Rat
Research Areas
Breast Cancer, Cardiovascular, Developmental Biology, Immunology, Cytokine Signaling, Defective Intrinsic Apoptosis, Infectious Disease, MAPK Signaling, PD-1 blockade immunotherapy, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
JUN
Positive Control
HEK293, HeLA or A-431 cells; Human normal lung or bladder tissue.
Immunogen
c-Jun protein phosphorylated at T91 and T93
Alternate Names
Transcription factor Jun, Activator protein 1, Proto-oncogene c-Jun, Transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, Activator protein 1(API); cJun; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; Jun proto oncogene; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; VV jun sarcoma virus 17 oncogene homolog; V-jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog

Database Links

Entrez Gene ID
3725
SwissProt
P05412

Additional Information

Clone
C-J 4C4/1
Chromosome Location
1p32.1
Mol. Weight of Antigen
39kDa

Related Antibodies

View All JUN Antibodies

Functions

  • Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Key References

  • Magrisso, I.J., et al. 1993. Lab Invest. 69(6):674-81.
  • Li, C., et al. 2016. Herz 41: 715-724.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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