Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Recombinant Neuronal-Nuclei (NeuN) (Neuronal Marker) Antibody [NeuN/7071R]

In Stock
Catalog Number Formulation Size Price
146713-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
146713-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
146713-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
Positive Control
Human brain tissue.
Immunogen
Synthetic peptide corresponding to residues within aa 30-60 of human NeuN protein (exact sequence is proprietary)
Alternate Names
RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, FLJ56884; FLJ58356; fox1 homolog C; Fox3; FOX3NeuN; hexaribonucleotide binding protein 3 (HRNBP3); neuronal nuclei; Rbfox3; RNA binding protein, fox 1 homolog (C. elegans) 3

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
NeuN/7071R
Chromosome Location
17q25.3
Mol. Weight of Antigen
38kDa

Functions

  • Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).

Key References

  • Edgar MA, et al. Arch Pathol Lab Med.2008; 132:500-9.
  • Ulbright TM, et al. Mod Pathol.2010; 23:972-80.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Neuronal-Nuclei (NeuN) (Neuronal Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK