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Nectin 4 / Nectin Cell Adhesion Molecule 4 / PVRL4 Antibody [NECT4/7271]

In Stock
Catalog Number Formulation Size Price
81607-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
81607-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
81607-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Homologous to the poliovirus receptor (PVR/CD155), the nectin immunoglobulin superfamily comprises four known isoforms (-1, -2, -3, and -4). The ectodomain of nectin family members comprises three Ig-like domains (V, C, C). Nectins localize at the adherens junctions (AJ) in epithelial and endothelial cells where they serve as adhesion molecules. Actin-based AJs play a role in mechanical adhesion, cellular morphogenesis and cellular differentiation. Nectin associates with the actin cytoskeleton through its interaction with the actin filament-binding protein afadin. Nectin 4 and afadin co-localize at cadherin-based adherens junctions in MDCKII epithelial cells. Nectin 4 and nectin 3 share a common binding region in the V domain of Nectin 1 and thus compete for Nectin 1 binding. The Nectin 3/4 binding domain maps to the C-C’-C”-D 尾 strands of the V domain of Nectin 1. Unlike other nectins, which are more widely expressed, Nectin 4 is mainly expressed in the placenta.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Adherens junction, Cell junction, Cell membrane, Secreted
Gene Name
Positive Control
Human tonsil, placenta or breast.
Immunogen
Recombinant fragment (around aa1-200) of human NECTIN4 protein (exact sequence is proprietary)
Alternate Names
Nectin-4, Ig superfamily receptor LNIR, Nectin cell adhesion molecule 4, Poliovirus receptor-related protein 4, Ig superfamily receptor LNIR family; Nectin 4; poliovirus receptor-related 4; Processed poliovirus receptor-related protein 4; PRR4; PVRL4

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
NECT4/7271
Chromosome Location
1q23.3
Mol. Weight of Antigen
66kDa

Functions

  • Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.
  • (Microbial infection) Acts as a receptor for measles virus.

Key References

  • Reymond, N., et al. 2001. Nectin 4/PRR4, a new afadin-associated member of the nectin family that trans-interacts with Nectin 1/PRR1 through V domain interaction. J. Biol. Chem. 276: 43205-43215.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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