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Formalin-fixed, paraffin-embedded human Stomach stained with MUC5AC Mouse Monoclonal Antibody (45M1).
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Mouse Monoclonal Antibody (45M1).
Formalin-fixed, paraffin-embedded Rat Stomach stained with MUC5AC Mouse Monoclonal Antibody (45M1).
SDS-PAGE Analysis Purified MUC5AC Mouse Monoclonal Antibody (45M1). Confirmation of Integrity and Purity of Antibody
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with Biotinylated MUC5AC Mouse Monoclonal Antibody (45M1).
SDS-PAGE Analysis of Purified Mucin-5AC Recombinant Mouse Monoclonal Antibody (45M1). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
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