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MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody [HMPV]

Additional information

Catalog Number: 4582-MSM14
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4582-MSM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4582-MSM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4582-MSM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV).

MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV). Confirmation of Integrity and Purity of Antibody

MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody - Image 3

Flow Cytometric Analysis of PFA fixed MCF-7 cells. MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (HMPV); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red)

MUC1 Antibody in Western Blot (WB)

MUC1 Antibody in Western Blot (WB)

Western Blot Analysis of Human kidney and Human Lung tissue lysates using MUC-1 Mouse Monoclonal Antibody (HMPV).

  • Flow
  • IF
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Clone
HMPV
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cytoplasm, Nucleus, Secreted
Gene Name
MUC1
Positive Control
Human kidney, lung, Colon, endometrial carcinoma., MCF-7 or MDA-231 cells. Breast, Ovarian
Immunogen
Human breast cancer cell line ZR-75 cells
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate on Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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Reactivity:

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Additional information

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Reactivity:

Gene:

Host:

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Additional information

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Gene:

Host:

Reactivity:

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  • WB

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Gene:

Host:

Reactivity:

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Other Information

Functions
  • The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
  • The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Key References
  • Xing PX,Prenzoska J,McKenzie IF. Mol Immunol.1992 May;29(5):641-50.
  • Devine PL., et al. Tumour Biol. 1992; 13(5):268-277.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4582
SwissProt
P15941
Chromosome Location
1q22
Mol. Weight of Antigen
265-400kDa
Alternate Names
Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

Summary

This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein.The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This MAb reacts with both normal and malignant epithelia of various tissues including breast and colon.

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