Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Mouse Monoclonal Antibody (ECM1/792).

SDS-PAGE Analysis Purified IgA Secretory Component Mouse Monoclonal Antibody (ECM1/792). Confirmation of Purity and Integrity of Antibody.

Printable Datasheet

IgA Secretory Component / ECM1 [ECM1/792]

Name: IgA Secretory Component / ECM1
SKU: 1893-MSM2
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Product Properties & Targets

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence

Species Reactivity

Human, Rat

Isotype

IgG1

Cellular Localization

Extracellular matrix, Extracellular space, Secreted

Light Chain

Kappa

Gene Name

ECM1

Positive Control

Lung, or Breast tumor., Stomach

Immunogen

Recombinant full-length human ECM1 protein

Alternate Names

Extracellular matrix protein 1, Secretory component p85, ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD

Database Links

Entrez Gene ID

1893

Unigene

81071

SwissProt

Q16610

Additional Information

Clone

ECM1/792

Human Gene Symbol

ECM1

Chromosome Location

1q21.2

Mol. Weight of Antigen

~80kDa

Functions

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
,Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Kuhn, L.C. and Kraehenbuhl, J.P. 1980. J. Biol. Chem. 254: 11072-11081.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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