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Human Nucleolar Antigen (Marker For Human Cells) Antibody [NM95]

Additional information

Catalog Number: MSM2-95-CF488-100T
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
MSM2-95-CF488-100T
Purified Ab conjugated to CF488
0.5ml at 100ug/ml
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Nucleolar Antibody in Flow Cytometry (FC)

Nucleolar Antibody in Flow Cytometry (FC)

Flow Cytometric Analysis of human Nucleolar Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled human Nucleolar Monoclonal Antibody (NM95).

Nucleolar Antibody in SDS-PAGE

Nucleolar Antibody in SDS-PAGE

SDS-PAGE Analysis Purified Nucleolar Mouse Monoclonal Antibody (NM95). Confirmation of Purity and Integrity of Antibody

Nucleolar Antibody in Flow Cytometry (FC)

Nucleolar Antibody in Flow Cytometry (FC)

Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolar Mouse Monoclonal Antibody (NM95) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Nucleolar Antibody in Immunofluorescence (IF)

Nucleolar Antibody in Immunofluorescence (IF)

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CF488-labeled human Nucleolar Monoclonal Antibody (NM95).

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Clone
NM95
Isotype
IgG1
Cellular Localization
Nucleolus
Gene Name
N/A
Positive Control
Any human cells. Tissues.
Immunogen
Nuclei of myeloid leukemia biopsy cells
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • N/A
Key References
  • Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
Not Known
SwissProt
N/A
Chromosome Location
Not Known
Mol. Weight of Antigen
Not Known
Alternate Names
N/A, N/A, Not Known

Summary

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

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