Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

HES5 / bHLHb38 / Hes family bHLH transcription factor 5 Antibody [HES5/8035]

In Stock
Catalog Number Formulation Size Price
388585-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
388585-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
388585-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The Drosophila Hairy and Enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homologues of Drosophila Hairy and Enhancer of split are the HES gene family members, HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system. HES6 is produced in brain as well as in the limb buds of developing embryos.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Cell surface
Gene Name
Immunogen
Recombinant fragment of human HES5 protein (exact sequence is proprietary)
Alternate Names
transcription factor HES-5, bHLHb38 antibody; Class B basic helix-loop-helix protein 38 antibody; Hairy and enhancer of split 5 antibody; Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody; HES 5 antibody; hes family bHLH transcription factor 5 antibody; Hes5 antibody; HES5 protein antibody; HES5_HUMAN antibody; MGI:104876 antibody; Transcription factor HES-5 antibody, bHLHb38

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
HES5/8035
Chromosome Location
1p36.32
Mol. Weight of Antigen
41kDa

Functions

  • Transcriptional repressor of genes that require a bHLH protein for their transcription.

Key References

  • Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004;24(9):3860-73.
  • Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol. 2001;166(1):197-206.
  • Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004;78:6995-7003.

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “HES5 / bHLHb38 / Hes family bHLH transcription factor 5 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK