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DRAP1 / NC2 alpha Antibody [PCRP-DRAP1-1A8]

In Stock
Catalog Number Formulation Size Price
10589-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10589-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10589-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

NC2 (negative cofactor 2) is a dimeric histone-fold complex that represses RNA polymerase II transcription through binding to TBP and inhibiting the transcription factors TFIIA and TFIIB. NC2 consists of two subunits, termed NC2a and NC2β, and these subunits dimerize and bind to TBP-promoter complexes via histone fold domains of the H2A-H2B type. NC2 associates with promoters in a manner that correlates with transcriptional activity and with occupancy by basal transcription factors. NC2 binds directly to DNA, and the binding of NC2 to TBP-promoter complexes affects the conformation of DNA, and results in the inhibition of TFIIB.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2b /
Cellular Localization
Nucleus. Cytoplasm.
Gene Name
Positive Control
HeLa or MCF-7 cells. Human brain|testis or smooth muscle.
Immunogen
Recombinant human DRAP1 protein
Alternate Names
Dr1-associated corepressor, Dr1-associated protein 1, Negative cofactor 2-alpha, DR1 associated corepressor; DR1 associated protein 1 (negative cofactor 2 alpha); DR1 associated protein 1; DRAP 1; Drap1; NC2 alpha; Negative co-factor 2-alpha

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-DRAP1-1A8
Chromosome Location
11q13.1
Mol. Weight of Antigen
22.35Da

Functions

  • The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own.

Key References

  • Blackshaw S, et al. Nature methods15.5 (2018 May): 330-338.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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