Desmoglein-3 (Squamous Cell Marker) [DSG3/2796]

Clear

Summary

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.

Product Properties & Targets

Host
Applications
Species Reactivity
Isotype
Cellular Localization
Cell junction, Cell membrane, Desmosome
Light Chain
Gene Name
Positive Control
A431 cells. Skin or Lung Squamous Cell Carcinoma (SCC).
Immunogen
Recombinant fragment (around aa 379-491) human DSG3 protein (exact sequence is proprietary)
Alternate Names
Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, 130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
DSG3/2796
Human Gene Symbol
DSG3
Chromosome Location
18q12.1
Mol. Weight of Antigen
130kDa

Functions

  • Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Known Applications & Suggested Dilutions

  • ELISA (For coating, order Ab without BSA)
  • Western Blot (1-2ug/ml)
  • Optimal dilution for a specific application should be determined.

Key References

  • Savci-Heijink CD, et al. The role of desmoglein-3 in the diagnosis of squamous cell carcinoma of the lung. Am J Pathology 2009; 174(5):1629-37.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Desmoglein-3 (Squamous Cell Marker)”

Your email address will not be published. Required fields are marked *

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies