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CLEC9A / DNGR1 (Dendritic Cell Marker) Antibody [8F9]

In Stock
Catalog Number Formulation Size Price
283420-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
283420-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
283420-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

This MAb recognizes a protein of 27kDa, identified as CLEC9A (C-type lectin domain family 9 member A). Its epitopes maps in aa 50-110. The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin- like receptor (CTLR) that acts as an activation receptor.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Membrane
Gene Name
Positive Control
MCF-7 cells (FACS/IF).
Immunogen
RBL-2H3 cells expressing human DNGR-1 fused to an HA epitope
Alternate Names
C-type lectin domain family 9 member A, C-type lectin domain family 9 member A (CLEC9A); DNGR1

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
8F9
Chromosome Location
12p13.2
Mol. Weight of Antigen
27kDa

Functions

  • Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

Key References

  • Drickamer, K. 1999. Curr. Opin. Struct. Biol. 9: 585-590.
  • Huysamen, C., et al. 2008. J. Biol. Chem. 283: 16693-16701

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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