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Chromogranin A / CHGA (Neuroendocrine Marker) Antibody [CGA/414]

Additional information

Catalog Number: 1113-MSM1-CF488-100T
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
1113-MSM1-CF488-100T
Purified Ab conjugated to CF488
0.5ml at 100ug/ml
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Chromogranin A Antibody in SDS-PAGE

Chromogranin A Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Chromogranin A Mouse Monoclonal Antibody (CGA/414). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Clone
CGA/414
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
CHGA
Positive Control
Adrenal gland, thyroid, bowel, pancreas or pheochromocytoma. PC12 cells.
Immunogen
Recombinant human chromogranin A protein
Storage & Stability
Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Strongly inhibits glucose induced insulin release from the pancreas.
  • Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172, PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).
  • Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Key References
  • Konecki DS et. al. J Biol Chem 1987;262:17026-30.
  • Lloyd RV et. al. Am J Pathol 1988; 130:296-304.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1113
SwissProt
P10645
Chromosome Location
14q32.12
Mol. Weight of Antigen
68-75kDa
Alternate Names
Chromogranin-A | Vasostatin-1 | Vasostatin-2 | EA-92 | ES-43 | Pancreastatin | SS-18 | WA-8 | WE-14 | LF-19 | Catestatin | AL-11 | GV-19 | GR-44 | ER-37 | GE-25 | Serpinin-RRG | Serpinin | p-Glu serpinin precursor, Pituitary secretory protein I, Vasostatin I, Vasostatin II, SL21, Beta-Granin; CGA; CHGA; Chromogranin A Parathyroid Secretory Protein 1; ER-37; Pancreastatin; Parastatin; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I; Vasostatin I or II

Summary

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

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