Flow Cytometric Analysis of PFA-fixed HeLa cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (green). CF640A-phalloidin (red).
Flow Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
SDS-PAGE Analysis Purified CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) Confirmation of Purity and Integrity of Antibody.
Flow Cytometric Analysis of PFA-fixed U87 cells CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4)followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunofluorescence Analysis of U87 cells using CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometric Analysis of PFA-fixed MCF-7 cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
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