Flow Cytometric Analysis of PFA-fixed HeLa cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunofluorescence Analysis of PFA-fixed HeLa cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (green). CF640A-phalloidin (red).

Flow Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

SDS-PAGE Analysis Purified CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) Confirmation of Purity and Integrity of Antibody.

Flow Cytometric Analysis of PFA-fixed U87 cells CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4)followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunofluorescence Analysis of U87 cells using CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometric Analysis of PFA-fixed MCF-7 cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Analysis of Protein Array containing more than 19,000 full-length human proteins using CELF2 Mouse Monoclonal Antibody (PCRP-CELF2-1E4). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Printable Datasheet

CELF2 / CUGBP2 (RNA-binding protein) [PCRP-CELF2-1E4]

Name: CELF2 / CUGBP2 (RNA-binding protein)
SKU: 10659-MSM1
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Properties & Targets

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence

Species Reactivity

Human

Isotype

IgG2b

Cellular Localization

Cytoplasm, Nucleus

Light Chain

Kappa

Gene Name

CELF2

Positive Control

HeLa or MCF-7 cells.

Immunogen

Recombinant full-length human CELF2 protein

Alternate Names

CUGBP Elav-like family member 2, Bruno-like protein 3, CUG triplet repeat RNA-binding protein 2, CUG-BP- and ETR-3-like factor 2, ELAV-type RNA-binding protein 3, Neuroblastoma apoptosis-related RNA-binding protein, RNA-binding protein BRUNOL-3, ETR3 ETR-3 NAPOR CELF-2 CUGBP2 BRUNOL3 CUG-BP2

Database Links

Entrez Gene ID

10659

Unigene

309288

SwissProt

O95319

Additional Information

Clone

PCRP-CELF2-1E4

Human Gene Symbol

CELF2

Chromosome Location

10p14

Mol. Weight of Antigen

54kDa

Functions

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats (By similarity). May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA (PubMed:28431233).

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Optimal dilution for a specific application should be determined.

Key References

New J, Subramaniam D, Ramalingam S, et al. Pleotropic role of RNA binding protein CELF2 in autophagy induction.Mol Carcinog. 201958(8)1400-1409. doi10.1002/mc.23023
Wang J et al. miR-615-3p promotes proliferation and migration and inhibits apoptosis through its potential target CELF2 in gastric cancer.Biomed Pharmacother. 2018101406-413.doi10.1016/j.biopha.2018.02.104

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05 BSA 0.05 azide. Also available WITHOUT BSA azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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